SONG HaiHong, BI Ying, HE LuanYing, YAN PeiLi, AN WenLin, YU ChangYuan, WANG ShiHui
Triptolide and celastrol are the main bioactive components of Tripterygium wilfordii Hook F and have many potential pharmacological effects and toxicities. Neuroinflammation is a prominent feature shared by various neurodegenerative diseases. Microglial activation is the principal player in neuroinflammation of the central nervous system. In this work, triptolide and celastrol were used to treat microglia in order to investigate their effects on microglia proliferation and cell membrane integrity, and triptolide was used to treat microglia to investigate its effect on M1 and M2 polarization. The results showed that after treatment of HMO6 microglia with celastrol (with a concentration of 10-4 mol/L), the inhibition of cell proliferation was higher than that of the control group, reaching (71.91±16.28)% (P<0.01, n=5), and the release of lactate dehydrogenase (LDH) (with a celastrol concentration of 10-5 mol/L) was significantly increased by (8.58±1.56)% (P<0.01, n=4). Compared with the control group, after treatment of HMO6 microglia with triptolide, there was no significant effect on cell membrane integrity, but when the triptolide concentration is 10-5 mol/L the cell proliferation inhibition was as high as (94.31±0.62)% (P<0.01, n=5). Compared with the lipopolysaccharide (LPS) group, when the triptolide concentration is 10-7 mol/L the release of tumor necrosis factor-α (TNF-α) was significantly reduced to (264.83±64.25) pg/mL (P<0.01, n=3) and the release of interleukin-10 (IL-10) was significantly decreased to (63.48±16.79) pg/mL (P<0.05, n=3).
