Preparation of multienzyme complexes and their applications in cascade reactions

CHEN BiQiang;CUI CaiXia;XIE Rong;TAN TianWei

Journal of Beijing University of Chemical Technology ›› 2014, Vol. 41 ›› Issue (5) : 1-8.

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Journal of Beijing University of Chemical Technology ›› 2014, Vol. 41 ›› Issue (5) : 1-8.
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Preparation of multienzyme complexes and their applications in cascade reactions

  • CHEN BiQiang;CUI CaiXia;XIE Rong;TAN TianWei
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Abstract

In bio-catalytic reactions, many cascade reactions need two or more enzymes to catalyze the reaction. In recent years, multi-enzyme reactions have begun to replace single enzyme and cell catalyzed reactions, and have becomes an important research direction in the development of enzyme engineering. In this paper, the methods and theories of preparation of multi-enzyme complexes and their applications in cascade reactions are reviewed. Methods of preparation of supramolecular enzymes discussed include undirected immobilization and directed gene fusion, cellulosome scaffolds and DNA scaffolds.

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CHEN BiQiang;CUI CaiXia;XIE Rong;TAN TianWei. Preparation of multienzyme complexes and their applications in cascade reactions[J]. Journal of Beijing University of Chemical Technology, 2014, 41(5): 1-8

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