Metabolic network balanced depends on a novel pathway using glycerol to produce 1,2- propanediol in Escherichia coli

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Journal of Beijing University of Chemical Technology ›› 2014, Vol. 41 ›› Issue (4) : 83-88.

生物技术与环境工程

SHEN XiaoLin;YUAN QiPeng

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Inexpensive glycerol has been used as a carbon source to produce 1, 2-propanediol by E. coli. We constructed a novel metabolic pathway using glycerol to produce 1, 2-propanediol in E. coli by knocking out competing acetate metabolic pathways. By enhancing the upstream metabolic pathway from glycerol to dihydroxyacetone phosphate, and balancing the metabolic network, carbon flux and energy, knockout strains ΔtpiA gave the best fermentation results, with the titer of 1, 2-propanediol being 1.3 g/L and the yield reaching 0.21g/g.

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SHEN XiaoLin;YUAN QiPeng. Metabolic network balanced depends on a novel pathway using glycerol to produce 1,2- propanediol in Escherichia coli[J]. Journal of Beijing University of Chemical Technology, 2014, 41(4): 83-88

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Received	Revised	Published
2014-04-14	1900-01-01	2014-07-20
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2014-07-20

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