Enhanced production of pyrroloquinoline quinone expressed from Escherichia coli via adding signal peptide

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Journal of Beijing University of Chemical Technology ›› 2013, Vol. 40 ›› Issue (s1) : 32-35.

生物技术与环境工程

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The signal peptide gene pelb from vector pET-22b was inserted into pET-28a-pqq, resulting in a new vector pET-28a-pqq. Through transformation and screening, a recombinant E.coli BL21(pET-28a-pqq) was acquired to express pyrroloquinoline quinone (PQQ). This strain produced 40.73mg/L of PQQ, which was 24.36% enhancement in comparison with the control strain E.coli BL21(pET-28a-pqq). This result implied the involvement of signal peptide in PQQ secretion or biosynthesis. Besides, both strains showed high PQQ productivity and yield on a biomass basis in the first 3h of fermentation. In particular, E.coli BL21(pET-28a-pqq) demonstrated an increase in PQQ production and active cell growth after 21h, indicating the synergistic relationship between PQQ biosynthesis and biomass.

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Enhanced production of pyrroloquinoline quinone expressed from Escherichia coli via adding signal peptide[J]. Journal of Beijing University of Chemical Technology, 2013, 40(s1): 32-35

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Received	Revised	Published
2013-05-15	1900-01-01	2013-12-20
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2013-12-20

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