Screening of Aspergillus awamori for the biosynthesis of genipin

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Journal of Beijing University of Chemical Technology ›› 2011, Vol. 38 ›› Issue (3) : 81-86.

生物技术与环境工程

FU YanShuai;ZHANG Peng;CHEN Chang

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A strain of fungus FYS-9 for producing genipin from β-glucosidase was screened from soil. This strain was identified as Aspergillus awamori according to its morphology characteristics and 18S rDNA gene sequence. Strain FYS-9 was utilized as the original strain to obtain another isolated mutant 70C-3. The β-glucosidase activity of strain 70C-3 was 36.15IU/mL, an improvement of 39.4% compared with the original strain. The optimal conditions were found to be fermentation for 24 h with addition of 1.5% geniposide at 30℃ with shaking at 150 r/min. The conversion of geniposide reached 97.7% and the yield of genipin reached 8.5g/L.

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FU YanShuai;ZHANG Peng;CHEN Chang. Screening of Aspergillus awamori for the biosynthesis of genipin[J]. Journal of Beijing University of Chemical Technology, 2011, 38(3): 81-86

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Received	Revised	Published
2010-12-26	1900-01-01	2011-05-20
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2011-05-20

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