Kinetics of formation of isoascorbyl palmitate by lipase-catalyzed esterification

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Journal of Beijing University of Chemical Technology ›› 2007, Vol. 34 ›› Issue (s2) : 95-98.

生物科学与技术

XU FengJie¹; TAN TianWei²

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The kinetics of the esterification reaction of isoascorbic acid with palmitic acid catalyzed by lipase from Candida sp. 99-125 have been studied in different organic solvents. The results showed that the initial rate v₀ is related to the ratio of molar concentrations, c_pal/c_iso. The value of v₀ initially increases with c_pal/c_iso and then reaches a maximum and remains constant at that value as c_pal/c_iso is increased further. The highest initial rate was obtained in acetone as solvent. The effects of varying temperature, shaking speed, initial water content and lipase concentration on the initial rate were investigated. On the basis of the kinetic model obtained, the reaction mechanism can be described by a PingPong model with v_m=5.686μmol/(min·g), and k'_m=0.092mmol/L. 

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XU FengJie¹; TAN TianWei². Kinetics of formation of isoascorbyl palmitate by lipase-catalyzed esterification[J]. Journal of Beijing University of Chemical Technology, 2007, 34(s2): 95-98

References

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Received	Revised	Published
2007-01-04	1900-01-01	2007-12-31
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2007-12-31

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