
Screening and fermentation of a directed S-1-phenyl alcohol-producing strain
CHEN HuaBao1;YANG Liu 2;ZHENG GuoJun1*
Journal of Beijing University of Chemical Technology ›› 2007, Vol. 34 ›› Issue (s2) : 106-110.
Screening and fermentation of a directed S-1-phenyl alcohol-producing strain
Screening of acetophenone reduction microorganisms has been carried out and one special strain was ultimately isolated from the soil when DL-1-phenyl alcohol was used as the only carbon source. This strain No. XC35-8 uses DL-1-phenyl alcohol as a growth inducer and gave high conversion and optical selectivity when it was used to reduce acetophenone to S-1-phenyl alcohol. The culture and reduction reaction parameters were investigated in detail and different cosubstrates were added in an attempt to improve the conversion. The results showed that the acetophenone conversion with strain No. XC35-8 reached 83.4% while the ee value of S-1-phenyl alcohol was as high as 99.5% when it was cultured for 30 hours at 30℃ with shaking at 200r/min and 0.1% (volume fraction) 1-phenyl alcohol as the inducer, 5g/L glucose as carbon source and 10g/L tryptone as nitrogen source followed by reaction for 24 hours with an acetophenone substrate concentration of 0.1% (volume fraction) with 6% (volume fraction) of 2-propanol as the co-substrate.
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