Screening and fermentation of a directed S-1-phenyl alcohol-producing strain

CHEN HuaBao1;YANG Liu 2;ZHENG GuoJun1*

Journal of Beijing University of Chemical Technology ›› 2007, Vol. 34 ›› Issue (s2) : 106-110.

PDF(942 KB)
Welcome to Journal of Beijing University of Chemical Technology, Today is July 27, 2025
Email Alert  RSS
PDF(942 KB)
Journal of Beijing University of Chemical Technology ›› 2007, Vol. 34 ›› Issue (s2) : 106-110.
生物科学与技术

Screening and fermentation of a directed S-1-phenyl alcohol-producing strain

  • CHEN HuaBao1;YANG Liu 2;ZHENG GuoJun1*
Author information +
History +

Abstract

Screening of acetophenone reduction microorganisms has been carried out and one special strain was ultimately isolated from the soil when DL-1-phenyl alcohol was used as the only carbon source. This strain No. XC35-8 uses DL-1-phenyl alcohol as a growth inducer and gave high conversion and optical selectivity when it was used to reduce acetophenone to S-1-phenyl alcohol. The culture and reduction reaction parameters were investigated in detail and different cosubstrates were added in an attempt to improve the conversion. The results showed that the acetophenone conversion with strain No. XC35-8 reached 83.4% while the ee value of S-1-phenyl alcohol was as high as 99.5% when it was cultured for 30 hours at 30℃ with shaking at 200r/min and 0.1% (volume fraction) 1-phenyl alcohol as the inducer, 5g/L glucose as carbon source and 10g/L tryptone as nitrogen source followed by reaction for 24 hours with an acetophenone substrate concentration of 0.1% (volume fraction) with 6% (volume fraction) of 2-propanol as the co-substrate. 

Cite this article

Download Citations
CHEN HuaBao1;YANG Liu 2;ZHENG GuoJun1*. Screening and fermentation of a directed S-1-phenyl alcohol-producing strain[J]. Journal of Beijing University of Chemical Technology, 2007, 34(s2): 106-110

References

[1]NAKAMURA K, MATSUDA T, OHAO A. Asymmetric synthesis of (S)-arylalkanols by microbial reduction[J]. Tetrahedron: Asymmetry, 1996, 7(10): 3021-3024. 
[2]刘惠, 林建平, 吴坚平, 等. 酵母生物合成S-腺苷-L-蛋氨酸的动力学研究[J]. 高校化学工程学报, 2004,
18(2):168-173. 
[3]HASEGAWA Y, ADACHI S, MATSUNO R. Asymmetric Reduction of Acetophenone by Immobilized Hansenula
capsulate Cells [J]. Journal of Fermantation and Bioengineering, 1998, 85(3): 322-327. 
[4]欧志敏, 吴坚平, 杨立荣, 等. 酵母细胞中辅酶Ⅰ含量的分光光度测定法[J]. 郑州轻工业学院学报: 自然科学版, 2005,
20(2): 20-22. 
[5]杨忠华, 邹少兰, 梅乐和, 等. 面包酵母在催化不对称合成中的应用[J]. 化学通报, 2004, 67(6):425-432. 
[6]PATEL R N, GOSWAMI A, CHU L, et al. Enantioselective microbial reduction of substituted acetophenones[
J]. Tetrahedron: Asymmetry, 2004, 15(8): 1247-1258. 
[7]王佳亮, 王建军, 杨柳, 等. 产苯乙醇胺醇脱氢酶菌种筛选与发酵条件研究[J]. 微生物学报, 2001, 41(5):635-641.

[8]徐寿昌. 有机化学[M]. 2版. 北京:高等教育出版社,1993. 
[9]DAHL A C, FIELDBERG M, MADSEN J. Baker's yeast: improving the D-stereoselectivity in reduction of
3-oxo esters[J]. Tetrahedron: Asymmetry,1999,10(3): 551-559.

PDF(942 KB)

3804

Accesses

0

Citation

Detail

Sections
Recommended

/