The conditions of fermentation for recombinant neurotoxins tranfering into P Pastoris GS 11.5 and their further purification procedures were investigated. An identification of the target protein was carried out with the SDS-PAGE electrophoresis and its productivity reached 350mg/L. After the purification procedures including the ultra filtration, ion-exchange chromatography and the gel filtration were performed, the purity of the product, which was analyzed by HPLC, was 92%.
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References
[1 ] Qian Youcun. Expression and characterization of two kinds of recombinant snake neurotoxins [ J ] . Chinese Journal of Biotechnology , 2000 ,5 (3) : 312 - 315
[2 ] Endo T , Tamiya N. Structure and function relationships of postsynaptic neurotoxins from snake venoms : In snake toxins[M]. New York : Pergamon Press , 1991 , 165 - 222
[3 ] 蔡勤,何志勇,龚毅. 中华眼镜蛇短链神经毒素cDNA 的克隆及在大肠杆菌中的表达[J ]. 遗传,1999 ,21(5) :1
[4 ] Anjou M C , Daugulis A J . A model2based feeding strategy for fed2batch fementation of recombinant pichia pastoris[J ] . Biotechnol Techniques , 1997 (11) : 865 - 868
[5 ] Maniatia T , Fritsch E F , Sambrook J . Molecular cloning a laboratory manual [ M ] . Beijing : Science Publishing company ,1993
[6 ] Invitrogen. Pchia expression kit [M] . California : Invitrogen , 2000
[7 ] 聂东宋,梁宋平,李敏. 外源蛋白在巴氏毕赤酵母中高效表达的策略[J ] . 吉首大学学报,2001 ,22 (3) :40 - 44
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Footnotes
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