Separation and purification of recombinant snake neurotoxins from Pichia pastoris

ZHANG Wei;CHEN Jin-chun*

Journal of Beijing University of Chemical Technology ›› 2005, Vol. 32 ›› Issue (6) : 20-22.

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Journal of Beijing University of Chemical Technology ›› 2005, Vol. 32 ›› Issue (6) : 20-22.
研究论文

Separation and purification of recombinant snake neurotoxins from Pichia pastoris

  • ZHANG Wei;CHEN Jin-chun*
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Abstract

The conditions of fermentation for recombinant neurotoxins tranfering into P Pastoris GS 11.5 and their further purification procedures were investigated. An identification of the target protein was carried out with the SDS-PAGE electrophoresis and its productivity reached 350mg/L. After the purification procedures including the ultra filtration, ion-exchange chromatography and the gel filtration were performed, the purity of the product, which was analyzed by HPLC, was 92%. 

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ZHANG Wei;CHEN Jin-chun*. Separation and purification of recombinant snake neurotoxins from Pichia pastoris[J]. Journal of Beijing University of Chemical Technology, 2005, 32(6): 20-22

References

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