Papain was immobilized on the mesoporous molecular sieve MCM-41 with the aid of a coupling agent,glutaraldehyde.The effects of immobilization conditions on the activity and the activity yield of the immobilized papain were studied.At the same time the continually operational stability of the immobilized papain was discussed.The results showed that the optimum conditions for immobilization were as follows:the concentration of the free enzyme, concentration of glutaraldehyde,time,temperature and pH were 20mg/g, 0.75%,2h,10~20℃ and 7.0, respectively. Under the optimum condition,the activity yield(%) of the immobilized papain was around 55%. The immobilized enzyme showed a good cotinually operational stability and its half-life up to 26 days.
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References
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Footnotes
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