对于工业发酵菌种肺炎克雷伯氏菌(Klebsiella pneumoniae ), 研究发现有两种消除其重组型质粒的有效方法，一种是连续传代培养，另一种是使用消除剂十二烷基硫酸钠(SDS)。对K. pneumoniae重组菌连续20代传代培养后，发现其质粒具有较高的消除率；而以0.2% SDS复合Ca²⁺处理K. pneumoniae重组菌，也能有效消除其重组型质粒，且该方法省却了反复的传代培养，能快速得到质粒消除菌，更具简便易操作性。消除了质粒的K. pneumoniae能再次接纳新的质粒，有效避免了因质粒不相容性带来的转化不成功，进而可用作宿主菌积累更多的生理性状。

Good physiological performance of industrial microbes is crucial for successful bioprocessing. This generally requires the elimination of plasmids. In order to eliminate the recombinant plasmids in Klebsiella pneumoniae , two methods have been investigated, namely subculturing and sodium dodecyl sulphate (SDS) treatment. After 20 generations of subculturing, recombinant K. pneumoniaeshowed a high plasmid elimination ratio, and the recombinant strain treated by 0.2% SDS coupled with Ca2+ also showed a high plasmid elimination ratio. After these treatments, the resulting plasmid-free strain was capable of uptaking new plasmids, which allows the host strain to accumulate other desirable physiological traits.