用偶联剂N-β-(氨乙基)-γ-氨丙基三甲氧基硅烷（AEAPTS）对网状多孔陶瓷表面改性，改性陶瓷用于辣根过氧化物酶的固定化。采用X射线能谱法对改性后的陶瓷表面元素进行了分析，结果表明AEAPTS向陶瓷表面引入了氨基；对陶瓷表面改性的研究结果表明，最适工艺条件为活化陶瓷的盐酸浓度2.5mol/L，改性剂AEAPTS质量分数15.0%，温度7.0℃，pH为4.5，反应时间3.5h。在此条件下，1g陶瓷与改性剂的最大结合量为2.13g。
Abstract
The surface of reticulated porous ceramics to be used for immobilization of enzymes was modified by the coupling agent N-β-aminoethyl-γ-aminoprop
yltrimethoxysilane (AEAPTS). Energy-dispersive X-ray spectrometry (EDS) showed
that AEAPTS had been successfully grafted on the surface of the ceramics. The surface of the ceramics was bound to the aminogroup of AEAPTS. The resultsindicated that the optimum conditions for grafting were as follows: concentration of hydrochloric acid used for activation of 2.5mol/L, mass fraction of AEAPTS of 15.0%, pH of 4.5, temperature of 70℃, and reaction time of 3.5h. Under these conditions, 1g of ceramics could be linked to the maximumamount of of coupling agent, namely 2.13g.
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