采用转基因pYBGA1酵母发酵葡萄糖及纤维二糖培养基，通过交叉实验研究了温度、酵母投入量对乙醇产率的影响；对木质纤维素进行爆破预处理，得到葡萄糖和纤维二糖的质量分数占50%预处理水解液，采用转基因pYBGA1酵母对预处理水解液发酵制备乙醇。结果表明，转基因pYBGA1酵母最佳发酵温度为28℃，最佳酵母投入量为10⁸mL^-1；当水解液中糖浓度为20g/L时，预处理水解液发酵得到乙醇的最大浓度可达6.8g/L。

he glucose and cellobiose was fermented by using pYBGA1 recombinant yeast，study the effects of the temperature and concentration of yeast-pYBGA1 on the ferment ethanol rate by using cross experiments; the lignocellulosic was pretreated through steam explosion pretreatment method, the glucose and cellobiose content in the pretreatment hydrolysate was 50%; the pretreatment hydrolysate was fermented by using yeastpYBGA1 to prepare the ethanol. Results shows that, the best fermentation temperature was 28℃ and the best to the ethanol productivity was 10⁸mL^-1; the highest concentration of ethanol by direct fermentation of mixed sugar in lignocellulosepretreatment hydrolysate is 6.8g/L when the suger concentration is 20g/L in the hydrolysate.