固定化混合菌种发酵玉米秸秆水解液的研究

李艳华;张 雯;房彩琴;张建刚;熊蓉春;魏 刚*

北京化工大学学报(自然科学版) ›› 2008, Vol. 35 ›› Issue (5) : 74-77.

PDF(899 KB)
欢迎访问北京化工大学学报(自然科学版),今天是 2025年7月11日 星期五
Email Alert  RSS
PDF(899 KB)
北京化工大学学报(自然科学版) ›› 2008, Vol. 35 ›› Issue (5) : 74-77.
材料科学与工程

固定化混合菌种发酵玉米秸秆水解液的研究

  • 李艳华;张 雯;房彩琴;张建刚;熊蓉春;魏 刚*
作者信息 +

Fermention of corn stalk hydrolysate by co-immobilized multi-microorganisms

  • LI YanHua;ZHANG Wen;FANG CaiQin;ZHANG JianGang;XIONG RongChun;WEI Gang
Author information +
文章历史 +

摘要

通过固定化混合菌种发酵法,研究了嗜单宁管囊酵母(Pachysolen tannophilus)和酿酒酵母(Saccharomyces cerevisiae)共固定于海藻酸钙中发酵玉米秸秆水解液工艺。研究结果表明,所得到的固定化混合菌种能同时利用玉米秸秆水解液中的木糖和己糖共同发酵,其效果明显优于游离混和菌种发酵,也明显优于S.cerevisiaeP.stipitis两种细胞共固定发酵。在发酵周期24h,初始pH值6.0,初始糖浓度118.42g/L的条件下,酒精转化率可达到0.4g/g糖。

Abstract

Pachysolen tannophilus and Saccharomyces cerevisiae yeast cells were co-immobilized by entrapment in marine alga calcium gel. Fermentation of corn sta
lk hydrolysate was studied using the immobilized yeast cells. It was found that the co-immobilized multi-microorganisms could utilize both hexoses and yloses
in the corn stalk hydrolysate. The fermentation results were superior to those
obtained with suspended multimicroorganisms, or separate samples of immobilized P. tannophilus or immobilized S. cerevisiae. The ethanol yield coeffic
ient reached a maximum of 0.4g/g under the optimal fermentation conditions which were determined on the basis of a single diathesis test to be as follows: fermentation time, 24h; concentration of glucose-xylose, 118.42g/L; fermenta
tion temperature, 30℃.

引用本文

导出引用
李艳华;张 雯;房彩琴;张建刚;熊蓉春;魏 刚*. 固定化混合菌种发酵玉米秸秆水解液的研究[J]. 北京化工大学学报(自然科学版), 2008, 35(5): 74-77
LI YanHua;ZHANG Wen;FANG CaiQin;ZHANG JianGang;XIONG RongChun;WEI Gang. Fermention of corn stalk hydrolysate by co-immobilized multi-microorganisms[J]. Journal of Beijing University of Chemical Technology, 2008, 35(5): 74-77

参考文献

[1] 冯玉杰,李冬梅. 混合菌群降解发酵木质纤维素类物质生产酒精的方法:中国,1896254A[P]. 20070117. 
[2] 王瑞明,关凤梅,马霞,等. 玉米秸秆水解与木糖酒精发酵的初步研究[J]. 酿酒,2002,29(1):56-57. 
[3] 邓立红,王艳辉,马润宇. 混和培养在酒精发酵中的研究进展[J]. 酿酒,2005,32(1):32-34. 
[4] Grootjen D R J, Meijlink H H M, van der Lans R G J M, et al. Cofermen
tation of glucose and xylose with immobilized Pichia stipitis and Saccharomyces cerevisiae[J]. Enzyme Microb Technol, 1990, 12: 860-864.
[5]Lebeau T, Jouenne T, Junter G A. Simultaneous fermentation of glucose
and xylose by pure and mixed cultures of Saccharomyces cerevisiae and Candida shehatae immobilized in a two-chambered bioreactor[J]. Enzyme and Microbial Technology, 1997, 21(4): 265-272. 
[6] Lebeau T, Jouenne T, Junter G A. Diffusion of sugars and alcohols through composite membrane structures immobilizing viable yeast cells[J]. Enzyme
and Microbial Technology, 1998, 22(6): 434-438. 
[7] 魏冬梅,张艳芳,张予林. 利用比色法测定葡萄酒的酒精度[J]. 食品工业,2001,4:45-46. 
[8] 杨小寒. 废弃植物纤维水解的研究[D]. 上海:东华大学环境科学与工程学院,2003. 
[9] Helle S, Cameron D, Lam J, et al. Effect of inhibitory compounds found in biomass hydrolysates on growth and xylose fermentation by a genetically eng
ineered strain of S.cerevisiae[J]. Enzyme and Microbial Technology, 2003, 33(6): 786-792. [ZK)]
[10]夏黎明,余世袁,丁红卫. 固定化休哈塔假丝酵母细胞发酵玉米秸秆水解液
的研究[J]. 林业化学与工业, 1994,14(1):51-54.

PDF(899 KB)

3129

Accesses

0

Citation

Detail

段落导航
相关文章

/