生物絮凝剂产生菌的筛选及性能研究

潘多涛; 刘桂萍; 刘长风

北京化工大学学报(自然科学版) ›› 2008, Vol. 35 ›› Issue (1) : 83-87.

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北京化工大学学报(自然科学版) ›› 2008, Vol. 35 ›› Issue (1) : 83-87.
生物科学与技术

生物絮凝剂产生菌的筛选及性能研究

  • 潘多涛; 刘桂萍; 刘长风

作者信息 +

Screening of microbe producing flocculant and characteristics of microbial flocculant

  • PAN DuoTao; LIU GuiPing; LIU ChangEeng
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文章历史 +

摘要

从林下土壤中筛选得一株产絮凝剂的细菌XMX-1(其产生的絮凝剂命名为XMX-1F);研究结果表明:玉米淀粉、玉米浆是廉价的碳氮源;探讨了在絮凝体系中,影响絮凝效果的因素。通过乙醇提取的方法,从发酵液离心后的上清液中获得絮凝剂粗产品7.685g/L;通过蒽酮反应、紫外扫描以及红外扫描等多种方法手段分析确认XMX-1F是以多糖为主的高分子絮凝剂,多糖含量为77.87%,且具有较高的热稳定性;在实际废水处理方面,XMX-1F对洗煤废水、泥浆废水以及活性炭悬液等有悬浮颗粒的废水处理效果明显,但脱色效果不明显,通过XMX-1F对模拟洗煤废水的应用研究表明,其应用到处理实际洗煤废水中的前景是可观的。

Abstract

In this study, a strain which had the highest flocculating activity was screened from soil, and named XMX-1 (the bioflocculant produced by XMX-1 named XMX-1F). The research showed that, the cornstarch and the corn steep liquor were chosen as carbon and nitrogen source; the flocculating conditions were studied. 7.685 g/L XMX-1F raw product was obtain form the supernatant by using ethanol. The XMX-1F was mainly composed of polysaccharide, which is determined by Anthrone reaction, ultraviolet spectroscope method and Infrared spectra. The polysaccharide content was 77.87%, in addition XMX-1F was more thermostable when treated at 100℃ for 30 min. In the treatment of high SS waste water, including coal-washing waste water, soil suspension and activate carbon power suspension, XMX-1F showed high flocculating activity, but the capability of decolorizing was not obviously. Study on XMX-1F flocculating coal-washing wastewater indicated the actual application potential of XMX-1F.

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潘多涛; 刘桂萍; 刘长风

.
生物絮凝剂产生菌的筛选及性能研究[J]. 北京化工大学学报(自然科学版), 2008, 35(1): 83-87
PAN DuoTao; LIU GuiPing; LIU ChangEeng
.
Screening of microbe producing flocculant and characteristics of microbial flocculant[J]. Journal of Beijing University of Chemical Technology, 2008, 35(1): 83-87

参考文献

[1] Takeda M, Kurane R. A protein bioflocculant produced by rhodococcus erythropolis[J]. Agric Biol Chem, 1991, 55(10): 2663 2664.
[2] 何宁,李寅,陈坚,等.生物絮凝剂的最新研究进展及其应用[J].微生物学通报,2005,32(2):104-108.
[3] 王兰.生物絮凝剂絮凝活性评价方法的探讨[犑].生物技术,2005,15(5):57-59.
[4]Breccia J D, Catro G R, Baigori M D, et al. Screening of xylanolytic bacteria using a colour plate method[J]. Journal of Applied Bacteriology, 1995, 78: 469 472.
[5] 李亚峰,刘铁成,曹丽丹.洗煤废水难处理的原因及处理方法研究[J].矿业安全与环保,1999(2):1-3.
[6] 马放,冯昊,李淑更,等.复合型微生物絮凝剂的絮凝作用[J].黑龙江科技学院学报,2004,14(3):140-144.
[7] 惠明,田青,马晓娜,等.枯草芽孢杆菌犅53产聚γ谷氨酸的絮凝特性[犑].生物技术,2006,16(4):68-70.
[8] 施抒伟,徐祖选.浑浊水样六价铬测定过程预处理[犑].福建环境,1999,16(2):34-36.
[9]Yokoi H, Arima T, Hirose J, et al. Flocculation properties of poly( y-glutamic acid) produced by Bacillus sutilis[J]. J Ferment Bioeng, 1996, 82:84 87.
[10]姚俊,徐虹.生物絮凝剂γ聚谷氨酸絮凝性能研究[J].生物加工过程,2004,2(1):35-39.
[11]康学军,曲见松,顾忠泽.白芷多糖的分析[J].分析化学研究简报,2006,34(4):533-535.
[12]马放,张金凤,远立江,等.复合型生物絮凝剂成分分析及其絮凝机理的研究[J].环境科学学报,2005,25(11):1491-1496.
[13]何玲玲,王新.苦丁茶冬青叶多糖的提取与鉴定[J].沈阳化工学院学报,2006,20(1):12-15.
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