采用在大鼠小肠内吸收药物的方法,对葛根素的肠吸收动力学进行了研究。利用UV双波长等吸收消去法测定小肠循环液中葛根素的含量,结果表明,对于50、100和200 μg/mL质量浓度的葛根素,在小肠内循环4h后相应的药物吸收速率常数(Ka)分别为0.021、0.028 和0.020 h-1,吸收率(Pa)分别为19.28%、21.78%和16.98%,在不同浓度下葛根素的吸收速率常数无显著差异(显著性水平因子P>0.05),该药物在肠道内的吸收呈现一级吸收动力学过程,其吸收机制为被动扩散。
Abstract
Absorption kinetics of puerarin in rats` intestines was studied. Puerarin in small intestinal solutions were determined by dual-λ UV. Puerarin solutions were circulated for 4 hours in the rat’s small intestine. At different concentrations of puerarin (50,100,200μg/mL), the intestinal absorption rate constants (Ka) were 0.021/h, 0.030/h, 0.020 /h-, and absorption percentage were19.28%、21.78%、16.98%, respectively. The intestinal absorption experiments of rats (in situ) showed the absorption of puerarin was a first-order process and the mechanism of absorption was passive diffusion transport.
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参考文献
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