酵母合成的15NL-色氨酸中N元素代谢通量的分析

刘占峰 ;袁其朋

北京化工大学学报(自然科学版) ›› 2006, Vol. 33 ›› Issue (4) : 88-90.

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北京化工大学学报(自然科学版) ›› 2006, Vol. 33 ›› Issue (4) : 88-90.
研究简报

酵母合成的15NL-色氨酸中N元素代谢通量的分析

  • 刘占峰 ;袁其朋&#;
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Metabolic flux analysis of elemental nitrogen for L-tryptophan biosynthesis in yeast

  • LIU Zhanfeng ;YUAN Qipeng*
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摘要

以葡萄糖为碳源,硫酸铵为氮源,15N邻氨基苯甲酸为前体物,利用酵母发酵合成15NL-色氨酸,是合成15NL-色氨酸的一种新方法。为了有效的利用含氮的化合物,实验过程中,采用15N标记的邻氨基苯甲酸为示踪剂,分析了N元素在15NL-色氨酸合成过程中的代谢通量。结果表明,93%外加的邻氨基苯甲酸,被转化为15NL-色氨酸。硫酸铵在促进菌体生长的同时,0.05%硫酸铵被用于合成邻氨基苯甲酸,并进一步被转化为L-色氨酸,5.95%硫酸铵被直接用于合成15NL-色氨酸。

Abstract

A new synthesis method for 15NL-tryptophan by yeast fermentation using glucose as carbon source, ammonium sulphate as nitrogen source, and 15N anthranilic acid as a precursor is described. In order to ensure efficient use of the nitrogen source compounds, the metabolic flux of elemental nitrogen was analyzed for L-tryptophan biosynthesis using the 15N anthranilic acid as a tracer in the experimental process. The results indicated that 93% of the L-tryptophan was derived from exogenous anthranilic acid. Ammonium sulphate contributed to the growth of the yeast and anthranilic acid was also synthesized from ammonium sulphate and subsequently converted into L-tryptophan (0.05% of the total yield). A further 5.95% of L-tryptophan was directly synthesized from ammonium sulphate.

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刘占峰 ;袁其朋 . 酵母合成的15NL-色氨酸中N元素代谢通量的分析[J]. 北京化工大学学报(自然科学版), 2006, 33(4): 88-90
LIU Zhanfeng ;YUAN Qipeng*
.
Metabolic flux analysis of elemental nitrogen for L-tryptophan biosynthesis in yeast [J]. Journal of Beijing University of Chemical Technology, 2006, 33(4): 88-90

参考文献

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