Using licorice as the raw material, and an alcoholic alkali solution as the extracting agent, the total flavonoids were extracted by an ultrasonic-assisted method. On the basis of single factor experiments, the optimum extraction conditions were determined by using Box-Benhnken design-response surface analysis, with the ethanol concentration, ammonia concentration, liquid to solid ratio, ultrasonic ation temperature, and ultrasonication time as the response variables, and the extraction ratio of total flavonoids from licorice as the response value. The results showed that the optimum extraction conditions were a ratio of liquid to solid of 11, volume fraction of ammonia of 1.0%, volume fraction of ethanol of 66%, ultrasonic time of 20min, and ultrasonic temperature of 74℃. The extraction ratio of total flavonoids was 2.15% under these conditions.
［1］韩雅慧, 陶宁萍. 甘草黄酮提取及其抗氧化能力测定方法研究进展[J]. 山西农业科学, 2010, 38(11): 89-93.
Han Y H, Tao N P. Progress in extraction methods of licorice root flavone and determination methods of its antioxidant activity[J]. Journal of Shanxi Agricultural Sciences, 2010, 38(11): 89-93. (in Chinese)
[2］朱飞娥, 田自华, 张子义. 甘草总黄酮微波法提取工艺的研究[J]. 畜牧与饲料科学, 2008(5): 1-2.
Zhu F E, Tian Z H, Zhang Z Y. A study on the extraction of total flavones from Glycyrrhiza uralensis using the technique of microwave[J]. Animal Husbandry and Feed Science, 2008(5): 1-2. (in Chinese)
[3］王应强, 张静, 段玉峰. 超声波提取甘草黄酮的工艺研究[J]. 粮油食品科技, 2006, 14(3): 42-43.
Wang Y Q, Zhang J, Duan Y F. Study on ultrasonicly extracting technology of flavons from the glycyrrhiza[J]. Food Science and Technology, 2006, 14(3): 42-43. (in Chinese)
[4］王勇, 赵海燕, 封琳, 等. 几种甘草黄酮含量测定方法的比较与评价[J]. 安徽农业科学, 2009, 37(28): 13593-13595.
Wang Y, Zhao H Y, Feng L, et al. Comparison and evaluation of methods for licorice flavonodis content determination[J]. Journal of Anhui Agricultural Sciences, 2009, 37(28): 13593-13595. (in Chinese)
[5］杨少娟, 赵海燕, 马永平, 等. 聚酰胺树脂层析纯化甘草黄酮的研究 [J]. 广东农业科学, 2011(16): 85-87.
Yang S J, Zhao H Y, Ma Y P, et al. Study on the separation and purification of licorice flavonoids with polyamide resin[J]. Guangdong Agricultural Sciences, 2011(16): 85-87. (in Chinese)
[6］李鸿飞, 卿德刚, 张娟, 等. 甘草渣中总黄酮清除·OH自由基能力的研究 [J]. 新疆医科大学学报, 2015, 38(2): 182-183/186.
Li H F, Qing D G, Zhang J, et al. The study of licorice flavonoids in licorice waste residue as a hydroxyl radical scavenger[J]. Journal of Xinjiang Medical University, 2015, 38(2): 182-183/186. (in Chinese)
[7］张娟, 倪慧, 卿德刚, 等. HPLC法指认甘草废渣中黄酮类成分及含量测定[J]. 中国中医药科技, 2012, 19(3): 233-234.
Zhang J, Ni H, Qing D G, et al. HPLC determination of flavonoids in licorice residue identified and content[J]. Chinese Journal of Traditional Medical Science and Technology, 2012, 19(3): 233-234. (in Chinese)
[8］林湖滨. 甘草黄酮对妊娠期糖尿病的疗效及安全性观察[J]. 现代诊断与治疗, 2015, 26(12): 2662-2663.
Lin H B. Licorice flavonoids on efficiency and safety of gestational diabetes mellitus［J］. Mod Diagn Treat, 2015, 26(12): 2262-2663. (in Chinese)
[9］唐浩国. 黄酮类化合物研究[M]. 北京: 科学出版社, 2009.
Tang H G. Study on flavonoids[M]. Beijing: Science Press, 2009. (in Chinese)
[10］张泽志, 韩春亮, 李成未. 响应面法在试验设计与优化中的应用[J]. 河南教育学院学报（自然科学版）, 2011, 20(4): 34-37.
Zhang Z Z, Han C L, Li C W. Application of response surface method in experimental design and optimization[J]. Journal of Henan Institute of Education(Natural Science Edition), 2011, 20(4): 34-37. (in Chinese)
[11］逯家辉, 姜鑫, 李昊龙, 等. 应用响应面法优化超声波法提取甘草中总黄酮的工艺[J]. 吉林大学学报（工学版）, 2008, 38(增刊2): 293-298.
Lu J H, Jiang X, Li H L, et al. Optimization of ultrasonic-assisted extraction of general flavonoids in glycyrrhiza uralensis using the response surface method[J]. Journal of Jilin University(Engineering and Technology Edition), 2008, 38(Suppl 2): 293-298. (in Chinese)
[12］邓引梅, 崔永明, 李唯, 等. 响应面法优化闪式提取甘草叶总黄酮工艺研究[J]. 化学与生物工程, 2008, 25(9): 44-47.
Deng Y M, Cui Y M, Li W, et al. Optimization for homogenate extraction of total flavonoids from glycyrrhiza leaves by response surface method[J]. Chemistry and Bioengineeing, 2008, 25(9): 44-47. (in Chinese)
[13］郭艳茹, 林洁. 乙醇浸提甘草中总黄酮的工艺研究[J]. 北方园艺, 2013(24): 158-161.
Guo Y R, Lin J. Research on the extraction technology of total flavonoids from licorice[J]. Northern Horticulture, 2013(24): 158-161. (in Chinese)
[14］汪河滨, 周忠波, 罗锋, 等. 甘草黄酮提取方法及抗氧化活性研究[J]. 时珍国医国药, 2008, 19(9): 2106-2107.
Wang H B, Zhou Z B, Luo F, et al. Study on extraction method of flavonoids from glycyrrhiza and its anti-oxida activity[J]. Lishizhen Medicine and Materia Medica Research, 2008, 19(9): 2106-2107. (in Chinese)
[15］何自强, 张惠玲, 张新欢. 超声波辅助乙醇-氨水提取茶皂素的工艺研究[J]. 湘潭大学自然科学学报, 2015, 37(2): 80-85.
He Z Q, Zhang H L, Zhang X H. Research on extracting technology of tea saponin by ultrasonic assisting alcohol-ammonia water[J]. Natural Science Journal of Xiangtan University, 2015, 37(2): 80-85. (in Chinese)
