过表达醛脱氢酶对 Klebsiella pneumoniae 合成3-羟基丙酸的影响

李莎1;曹宁1;葛喜珍2;田平芳1*

北京化工大学学报(自然科学版) ›› 2013, Vol. 40 ›› Issue (6) : 72-75.

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北京化工大学学报(自然科学版) ›› 2013, Vol. 40 ›› Issue (6) : 72-75.
生物技术和环境工程

过表达醛脱氢酶对 Klebsiella pneumoniae 合成3-羟基丙酸的影响

  • 李莎;曹宁;葛喜珍;田平芳*
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Effect of aldehyde dehydrogenase overexpression on 3-hydroxypropionic acid production in Klebsiella pneumoniae

  • LI Sha1;CAO Ning1;GE XiZhen2;TIAN PingFang1
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摘要

克隆了肺炎克雷伯氏菌(Klebsiella pneumoniae)及大肠杆菌(Escherichia coli)各自的醛脱氢酶基因puuCaldH,获得了重组肺炎克雷伯氏菌K.p(pET-PK-puuC)和K.p(pET-PK-aldH),这两株菌均能以甘油为唯一碳源生产3-羟基丙酸(3-HP)。发酵实验结果显示K.p(pET-PK-puuC)的甘油转化率和单位菌体产量与野生菌相比分别提高了135%和18%;K.p(pET-PK-aldH)的3-HP产量达1.43g/L,是K.p(pET-PK-puuC)的1.8倍,比野生菌提高了81%,甘油转化率和单位菌体产量比野生菌分别提高了150%和88%。

Abstract

The aldehyde dehydrogenase coding genes puuC and aldH from Klebsiella pneumoniae DSM 2026 and Escherichia coli K-12 respectively have been cloned, and two recombinant strains K.p(pET-PK-puuC)and K.p(pET-PK-aldH) were acquired. Upon glycerol induction, both strains could produce 3-hydroxypropionic acid (3-HP) using glycerol as the sole carbon source. Compared with the wild type strain, the recombinant strain K.p(pET-PK-puuC) showed increases of 135% and 18% in glycerol conversion ratio and yield on a biomass basis, respectively. The strain K.p(pET-PK-aldH) produced 1.43g/L of 3-HP, which is 1.8 times that produced by K.p(pET-PK-puuC), and demonstrated increases of 81%, 150% and 88% in 3-HP production, glycerol conversion ratio and yield on a biomass basis, respectively.

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李莎1;曹宁1;葛喜珍2;田平芳1*. 过表达醛脱氢酶对 Klebsiella pneumoniae 合成3-羟基丙酸的影响[J]. 北京化工大学学报(自然科学版), 2013, 40(6): 72-75
LI Sha1;CAO Ning1;GE XiZhen2;TIAN PingFang1. Effect of aldehyde dehydrogenase overexpression on 3-hydroxypropionic acid production in Klebsiella pneumoniae[J]. Journal of Beijing University of Chemical Technology, 2013, 40(6): 72-75

参考文献

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