制备色谱法在纯化甜茶苷工艺中的应用

吕鑫华;况鹏群;袁其朋*;郑桂清

北京化工大学学报(自然科学版) ›› 2012, Vol. 39 ›› Issue (5) : 92-97.

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北京化工大学学报(自然科学版) ›› 2012, Vol. 39 ›› Issue (5) : 92-97.
生物技术与环境工程

制备色谱法在纯化甜茶苷工艺中的应用

  • 吕鑫华;况鹏群;袁其朋*;郑桂清
作者信息 +

The use of preparative HPLC in the purification of rubusoside from Rubus suavissimus S. Lee leaves

  • LU XinHua;KUANG PengQun;YUAN QiPeng;ZHENG GuiQing
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摘要

研究了从甜茶叶中纯化甜茶苷的制备工艺。首先用沸水从甜茶叶中提取甜茶苷,提取3次,每次1 h。再利用HP-20大孔吸附树脂对甜茶苷粗提物进行预纯化,以90%乙醇为洗脱液,洗脱6倍柱体积;浓缩去除乙醇后,用D-941阴离子交换树脂脱色,水洗并干燥得到甜茶苷粗产品,纯度为68.6%。最后甜茶苷粗产品经制备色谱纯化后,得到纯度为97.9%的甜茶苷,色谱条件为流动相V甲醇 ∶V=70∶30,流速10mL/min,上样量200 mg。

Abstract

The preparative separation of rubusoside from the leaves of Rubus suavissimus S. Lee has been investigated. First, the leaves were extracted three times using boiling water, with an extraction time of 1 h. Second, HP-20 macroporous resins were used in the pre-purification procedure, with 6BV of 90% ethanol in water used as the eluent. Third, the solution was evaporated to remove ethanol and loaded onto pretreated D-941 anion-exchange resin for decoloration. Finally, preparative HPLC was used for further purification. Rubusoside with a purity of 97.9% was obtained from the 68.6% purity rubusoside extract after the preparative HPLC using 70% methanol in water as the mobile phase, a flow rate of 10 mL/min and a sample loading of 200 mg.

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吕鑫华;况鹏群;袁其朋*;郑桂清. 制备色谱法在纯化甜茶苷工艺中的应用[J]. 北京化工大学学报(自然科学版), 2012, 39(5): 92-97
LU XinHua;KUANG PengQun;YUAN QiPeng;ZHENG GuiQing. The use of preparative HPLC in the purification of rubusoside from Rubus suavissimus S. Lee leaves[J]. Journal of Beijing University of Chemical Technology, 2012, 39(5): 92-97

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