响应面法优化虎杖中反式白藜芦醇的提取工艺及抑菌活性

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北京化工大学学报（自然科学版） ›› 2010, Vol. 37 ›› Issue (4) : 108-112.

蔡杨柳;陈劲春*

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Optimization by response surface analysis of the extraction of trans-resveratrol from Polygonum cuspidatum and a study of its antibacterial effects

CAI YangLiu;CHEN JinChun

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采用响应面分析法优化了虎杖中反式白藜芦醇的提取工艺，其最优提取工艺条件为：以体积分数79%乙醇为溶剂，53℃回流提取1.1h；此条件下白藜芦醇提取量为5.912mg/g，与理论值5.918mg/g接近。产物经红外和质谱鉴定为反式白藜芦醇（纯度99.8%）。考察了经分离纯化后的反式白藜芦醇对大肠杆菌、金黄色葡萄球菌的体外抑菌作用，结果表明所得反式白藜芦醇具有抑菌活性，10h后质量浓度为0.125mg/mL的反式白藜芦醇对金黄色葡萄球菌的抑菌率为80%，而质量浓度为1.00mg/mL的反式白藜芦醇对大肠杆菌的抑菌率为40%。

Abstract

The optimal conditions for the extraction of trans-resveratrol from the herb Polygonum cuspidatum have been obtained by response surface analysis. When extracted with 79% ethanol for 1.1h at 53℃, the extracted content was as high as 5.912mg/g, close to the calculated value of 5.918mg/g. Infrared spectroscopy and electrospray ionization mass spectrometry confirmed that the product was trans-resveratrol. The material showed good antibacterial efficiency. The inhibitory ratio of trans-resveratrol on Staphylococcus aureus was 80% when the concentration was 0.125mg/mL, and the inhibitory ratio of trans-resveratrol on Escherichia coli was 40% when the concentration was 1.00mg/mL after 10 hours.

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蔡杨柳;陈劲春*. 响应面法优化虎杖中反式白藜芦醇的提取工艺及抑菌活性[J]. 北京化工大学学报（自然科学版）, 2010, 37(4): 108-112

CAI YangLiu;CHEN JinChun. Optimization by response surface analysis of the extraction of trans-resveratrol from Polygonum cuspidatum and a study of its antibacterial effects[J]. Journal of Beijing University of Chemical Technology, 2010, 37(4): 108-112

参考文献

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收稿日期	修回日期	出版日期
2010-03-22	1900-01-01	2010-07-20
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2010-07-20

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