含硫树脂的制备及在谷胱甘肽分离中的应用

赵睿1,2;王满意1,2;周鑫1,2;谭天伟*1,2

北京化工大学学报(自然科学版) ›› 2007, Vol. 34 ›› Issue (3) : 304-307.

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北京化工大学学报(自然科学版) ›› 2007, Vol. 34 ›› Issue (3) : 304-307.
生物科学与技术

含硫树脂的制备及在谷胱甘肽分离中的应用

  • 赵睿,;王满意,;周鑫,;谭天伟*,
作者信息 +

The preparation of resin containing sulf-group and their application in glutathione separation

  • ZHAO Rui1,2;WANG ManYi1,2;ZHOU Xin1,2;TAN TianWei1,2
Author information +
文章历史 +

摘要

利用大孔苯乙烯树脂改性合成含硫树脂,考察了修饰条件对谷胱甘肽吸附的影响。并对其在谷胱甘肽分离中的应用进行了研究。结果表明采用pH3.0的缓冲液进行吸附,自制的含硫树脂最大吸附容量可达22mg/g湿树脂,选择质量浓度为0.5%的NaOH溶液洗脱GSH,解吸率可达70%以上。采用真实发酵液进行分离纯化,收率为35%,纯度可达90%以上。

Abstract

Chloromethylated styrene-divinylbenzene resin has been modified with thioureaunder a variety of conditions and the adsorption and desorption of glutathione on the modified resins has been investigated. The optimal pH for adsorption was found to be 3.0, giving a maximum adsorption capacity of glutathione of 22mg/g wet resin. Recovery of glutathione reached 70% when 0.5%-1.5% NaOH solution was employed for desorption. Chromatography experiments indicated that GSH could be successfully purified from clarified broth, and that the resins have potential applications in GSH separation.

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赵睿1,2;王满意1,2;周鑫1,2;谭天伟*1,2. 含硫树脂的制备及在谷胱甘肽分离中的应用[J]. 北京化工大学学报(自然科学版), 2007, 34(3): 304-307
ZHAO Rui1,2;WANG ManYi1,2;ZHOU Xin1,2;TAN TianWei1,2. The preparation of resin containing sulf-group and their application in glutathione separation[J]. Journal of Beijing University of Chemical Technology, 2007, 34(3): 304-307

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