高效液相色谱分析L-瓜氨酸的研究

牟昳;袁其朋*;翁南梅; 东惠茹;

北京化工大学学报(自然科学版) ›› 2004, Vol. 31 ›› Issue (3) : 110-112.

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北京化工大学学报(自然科学版) ›› 2004, Vol. 31 ›› Issue (3) : 110-112.
研究简报

高效液相色谱分析L-瓜氨酸的研究

  • 牟昳;袁其朋*;翁南梅; 东惠茹;
作者信息 +

High performance liquid chromatographic analysis of L-citrulline

  • Mu Yi ;Yuan Qi-peng*;Weng Nan-mei;Dong Hui-ru;
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文章历史 +

摘要

文中建立了L-瓜氨酸的HPLC检测方法,实验应用2,4二硝基氟苯(DNFB)柱前衍生化法将L瓜氨酸衍生后在HPLC上进行定量分析,探讨和优化了L-瓜氨酸的HPLC分析色谱条件。研究结果表明,L 瓜氨酸样品检测线性范围为2~10μg ,相关因数r=0.9990,精密度为1.7%。该方法灵敏,重复性好,结果准确,分析迅速,能有效的指导L-瓜氨酸的发酵生产。

Abstract

A HPLC method for analyzing L citrulline was established and the HPLC chromatographic conditions such as the ultraviolet wavelength, column temperature, flow rate and proportion of the mobile phase were optimized. The linear relationship is very good ( r=0.9990) within the range of 2~10μg. This analysis method is sensitive and accurate for determining the concentration of L-citrulline and plays an instructive role in the production of L-citrulline.

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牟昳;袁其朋*;翁南梅; 东惠茹;. 高效液相色谱分析L-瓜氨酸的研究[J]. 北京化工大学学报(自然科学版), 2004, 31(3): 110-112
Mu Yi ;Yuan Qi-peng*;Weng Nan-mei;Dong Hui-ru;. High performance liquid chromatographic analysis of L-citrulline[J]. Journal of Beijing University of Chemical Technology, 2004, 31(3): 110-112

参考文献

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[6] 陈娅兰,付宜和,王国林,等. 十八种氨基酸注射液的HPLC 2 ,4-二硝基氟苯柱前衍生化法含量测定[J] . 药物分析杂志,1990 ,10 (3) :149
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